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. 2015 Oct 12;5:15074. doi: 10.1038/srep15074

Figure 3. Samples from 10 dpa soybean pods and seeds are negative for the presence of HPS.

Figure 3

(A) Bright field micrograph of transverse section through pod and developing seed, stained with Toluidine blue O. This is the typical orientation for sample sectioning. Bar = 20 μm. (B) Transmission electron micrograph of endocarp epidermis, showing a portion of the outer tangential wall and radial wall, after probing with monoclonal antibody to HPS and immunogold labelling. Bar = 2 μm. (C) Transmission electron micrograph of the palisade layer. The cytoplasm is dense with organelles and the vacuoles are still small and not yet coalesced into a single central vacuole. Bar = 500 nm. (D) Higher magnification view of endocarp epidermis after probing with monoclonal antibody to HPS and immunogold labelling, showing unlabelled deposits. Bar = 500 nm. ep, endocarp epidermis; f, funiculus; n, nucleus; p, pod wall; pa, endocarp parenchyma; s, seed; st, starch grain; v, vacuole; w, cell wall.