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. 2015 Oct 12;5:15074. doi: 10.1038/srep15074

Figure 5. Transmission electron microscopy of endocarp and seed coat showing HPS deposition.

Figure 5

Panels (A–G) show samples labeled with monoclonal antibody to HPS and immunogold; panel (H) stained with uranyl acetate and lead citrate, but not labeled with immunogold. (A) Deposits on surface of 26 dpa endocarp epidermis (bar = 2 μm); box indicates area enlarged in (B), showing heterogeneous surface deposits with gold label. (Bar = 500 nm.) (C) Endocarp epidermis showing labeled surface deposit. (Bar = 500 nm.) (D) Endocarp epidermis with nucleus (n) and vacuole (v), showing gold label localized to dense heterochromatic nuclear regions (arrowheads). (Bar = 500 nm.) (E) Endocarp epidermal cell with labeled nucleus. Large, unlabeled starch grains are present. (Bar = 500 nm.) (F) Endocarp epidermal cell showing gold label associated with rough endoplasmic reticulum (arrows) and golgi within cytoplasm; cell wall and vacuole also shown, but minimally labeled. Bar = 500 nm. (G) Seed coat palisade cells showing dense cytoplasm, electron-dense cuticle on surface and lack of gold label (Bar = 500 nm). (H) Detail of endocarp epidermal surface deposits revealing a fine reticulate network of moderately electron-dense material surrounding large electron-lucent bodies. (Bar = 100 nm). cu, cuticle; ep, endocarp epidermis; g, golgi body; hc, heterochromatic regions of nucleus; pl, palisade cells; n, nucleus; st, starch grain; v, vacuole; w, cell wall.