Figure 3.

Effect of mitochondrial inhibition on cellular glucose uptake kinetics. (A) Inhibition of the GLC consumption flux (V₂) allows analysis of GLC uptake (V₁). (B) Typical GLC uptake in cells pretreated with vehicle, PA, or AA for 30 min. The maximal rate of linear increase of these curves was used as a measure of V₁. (C) Mean (±SE) of the maximal rate of [GLC]_c increase as a function of [GLC]_ext in vehicle-treated cells and cells treated with PA or AA (n = 20 cells per condition; N = 4 independent experiments). A Hill equation was used for curve fitting (Vehicle: R² = 0.85; PA: R² = 0.68; AA: R² = 0.94). (D) Average value (±SD) of K_m for vehicle-, PA-, or AA-treated cells. (E) Similar to (D), but now for the cooperativity parameter n. (F) Similar to (D), but now for V_max. To see this figure in color, go online.