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. 2015 Oct 6;109(7):1472–1482. doi: 10.1016/j.bpj.2015.08.004

Figure 5.

Figure 5

Single molecule analyses of homodimers illustrate differences in KIF3 motors. (A–D) Single molecule run length and velocity (insets) histograms with representative kymographs for homodimeric KIF3 motors (mean ± SE). See also Fig. 4E. (A) KIF3CC data with a highlighted trace (solid arrow) representing a 1.28 μm run at 6 nm/s (scale bars: 3 min along x axis; 5 μm along y axis). See also Movie S5. (B) KIF3CCΔL11 data with a highlighted trace (arrowhead) representing a 2.4 μm run at 6 nm/s (scale bars: 3 min along x axis; 5 μm along y axis). See also Movie S6. (C) KIF3AA data, with a highlighted trace (open arrow) representing a 6.1 μm run at 288 nm/s (scale bars: 25 s along x axis; 5 μm along y axis). See also Movie S7. (D) KIF3BB data with a highlighted trace (∗) representing a 5.8 μm run at 409 nm/s (scale bars: 25 s along x axis; 5 μm along y axis). For KIF3BB the microtubule-motor complex was preformed with 0.5 mM MgAMP-PNP before activation by 1 mM MgATP. See also Movie S8. To see this figure in color, go online.