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. 2015 Aug 31;6(10):1075–1079. doi: 10.1021/acsmedchemlett.5b00254

Table 2. Kinetic Characterization of dGTP Misincorporation Opposite dT for RT (WT) and RT (K101P).

  Kd dGTP (μM) kpol dGTP (s–1) efficiency (kpol/Kd; s–1 μM–1) fidelityc
RT (WT) 780 ± 470 0.11 ± 0.02 1.4 × 10–4 ± 8 × 10–5 45000a
RT (K101P) 530 ± 110 0.39 ± 0.04 7.4 × 10–4 ± 1.7 × 10–4 3700b
a

Calculated using experimentally derived efficiencies of 6.4 s–1 μM– 1 and

b

2.8 s–1 μM–1 for correct incorporation (Table 1).

c

Fidelity is defined as the ratio of efficiencies for correct/incorrect incorporation for RT (WT) and RT (K101P), respectively.