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Determination of reversibility of MPO inhibition; ABAH (6 μM), SHA (100 μM), 1c (50 μM), and 2k (50 μM), all in DMSO (1% final) or DMSO alone (Control), were incubated with MPO bound to wells of an ELISA plate for 1 h in the presence of 50 μM H2O2. Wells were either washed to remove inhibitor (gray bars) or remained unwashed (black bars) prior to measuring MPO activity as in Methods, SI. Results show mean MPO activity (ΔA652 nm/min values ± SEM, n = 3).
