Figure 2. In silico analyses of Orc1.

(a) Multiple alignment of a BLAST search with ScOrc1^201–420 is shown on top using a colored rectangular pixel for each residue. A magnified alignment of the basic patch is shown below. The residues assigned to each color are indicated by the legend at left *, conserved residues. Full details of the alignment are shown in Supplementary Data S1. (b) Multiple alignment of the basic patch of eukaryotic Orc1 homologs, including representative model species. NCBI accession numbers are indicated on the right. Residues are colored as in panel (a). Histidine, though less basic than lysine or arginine, can play a crucial role for DNA binding at physiological pH¹⁹. *, Lys-362 and Arg-367 residues in ScOrc1 and equivalents in homologs.