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. 2015 Oct 12;5:14929. doi: 10.1038/srep14929

Figure 5. Affinities of ScORC for dsDNAs.

Figure 5

(a) EMSA of Cy5-labeled wild-type (WT) or mutant (A B2 B3) ARS1 DNA with wild-type or mutant ORC in the presence of competitor DNA. (b) Quantified results from panel (a) are shown. (c) EMSA of Cy5-labeled ARS306 or ARS609 DNA with wild-type or mutant ORC in the presence of competitor DNA. Wild-type (WT) or mutant (A B2 B3 or mut) ARS1 was also used as controls. (d) Quantified results from panel c are shown. (e,f) EMSA of Cy5-labeled DNA in the absence of competitor DNA. DNAs were the same as in panels (a,b).