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. 2015 Oct 12;5:14929. doi: 10.1038/srep14929

Figure 6. Affinities of Orc1301–400 for dsDNAs.

Figure 6

(a) Wild-type and mutant GH-tagged Orc1301–400 (WT and R367A; 0.5 μg each) were analyzed by 12% SDS-PAGE, followed by Coomasie Brilliant Blue staining. (b) EMSA of Cy5-labeled mutant (A− B2− B3−) ARS1 DNA with wild-type or mutant GH-Orc1301–400 in the absence of competitor DNA. (c) EMSA of Cy5-labeled wild-type ARS1 DNA with ORC hexamer or GH-Orc1301–400 in the presence of competitor DNA. (d) EMSA of Cy5-labeled wild-type (WT) or mutant (A− B2− B3−) ARS1 DNA with wild-type or mutant GH-Orc1301–400 in the presence of competitor DNA.