Figure 2.

Hypoxia Induces Lipid Droplets in the CNS

(A) Oil red O-stained neutral lipids in the CNS of a late-third instar larva (control genotype: w¹¹¹⁸) raised on a standard diet.

(B) LipidTOX stained neutral lipids in the CNS of a late-third instar larva raised on a standard diet. Neutral lipids (red) are prominent in the central brain and ventral nerve cord but do not overlap with neuroblast lineages (green: nab-Gal4 > CD8::GFP). Scale bar, 50 μm.

(C) Neutral lipids (LipidTOX, red) in the thoracic ganglion of the late third instar CNS accumulate in clusters of lipid droplets labeled with Lsd-2::YFP (green). Scale bars, 1 μm.

(D) Lipid droplets (LipidTOX) in the CNS of a mid-third instar (70 hr) larva fed on a standard diet increase during NR normoxia and become more numerous during NR hypoxia. The lipid droplet (LD) content of the wing disc does not change significantly during hypoxia. It is expressed as percentage of volume within the thoracic region of interest of the ventral nerve cord (white boxes, upper row) or the entire wing disc (white outlines, lower row). Scale bars, 50 μm.

(E) Lipid droplets increase ∼25-fold in normoxia and ∼80-fold in hypoxia in the CNS but do not significantly change in the wing disc. Note that fold change values in this figure are normalized to the 70 hr start points of Figure 2D.

See also Figure S2.