Figure 6.

Autophagy Block Causes α-syn Accumulation

(A) IF of Ifnb^–/– CN with inclusion body (arrow). Scale bar, 20 μm; 2 μm in insert.

(B) WB and quantified IOD bands of monomeric and high-molecular-weight (Mw) α-syn. Data are mean ± SEM, n = 3. ^∗p < 0.05 by unpaired Student’s t test.

(C) WB of Ifnb^+/+ CNs treated with 20 mM NH₄Cl.

(D) IF of CNs from Ifnb^–/– mice. Arrows, triple colocalizing vesicular structures; arrowheads, LC3B and phosphorylated (Ser129) α-syn-positive vesicular structures. Scale bars, 10 μm; 2 μm in inserts. See also Figure S6.