Table 1.
Kinetic constants of M (412 nm) and O (612 nm) intermediates for DDM-purified preparations of IAR and KR2 and IAR in LUVs in 100 mM NaCl or 100 mM KCl
| IAR DDM Na+ | IAR LUV Na+ | KR2 DDM Na+ | IAR DDM K+ | IAR LUV K+ | KR2 DDM K+ | |
|---|---|---|---|---|---|---|
| 412 nm | ||||||
| Rise1 | 0.033 ms | 0.025 ms | 0.039 ms | 0.033 ms (58%) | 0.246 ms (60%) | 0.037 ms (78%) |
| Rise2 | 3.09 ms (42%) | 3.10 ms (40%) | 5.38 ms (22%) | |||
| Decay1 | 0.75 ms (52%) | 0.70 ms (51%) | 0.40 ms (72%) | 294 ms | 89.10 ms | 215 ms |
| Decay2 | 6.0 ms (48%) | 3.6 ms (49%) | 1.4 ms (28%) | |||
| 612 nm | ||||||
| Rise1 | 0.75 ms (34%) | 0.58 ms (55%) | 0.70 ms | 6.80 ms (7%) | 36.40 ms | 180 ms |
| Rise2 | 2.5 ms (66%) | 2.1 ms (45%) | 90.0 ms (93%) | |||
| Decay | 17.8 ms | 15.9 ms | 8.7 ms | 470 ms | 184.3 ms | 550 ms |
Numbers in parentheses indicate the percentage of each phase in rise or decay.