Figure 4. Glycemic control in adult GPR3 mice.

(A–E) Hyperinsulinemic euglycemic clamps were performed in conscious, unrestrained 5-month-old male GPR3 WT and KO mice. Time course of blood glucose levels and glucose infusion rates (GIR; (A)) during the course of clamps; Glucose Infusion Rate (B), whole body glucose clearance (Rd; (C)), levels of hepatic glucose production (hGP; (D) were measured during the steady-state period of the clamp. (E) Glucose uptake into individual tissues: interscapular brown adipose tissue (iBAT), epididymal (epidyd. ad. t.), subcutaneous (subcut ad. t.) and perirenal adipose tissues (ad. t.), heart, liver, gastrocnemius muscle (gastrocnemius m.) and hypothalamus. Tissue uptake was determined as described in materials and methods. Values are mean ± s.e.m of n = 6–11 samples (except hypothalamus for which n = 5–7). ***P < 0.001 compared to age-matched WT littermates.