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. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Anal Chem. 2015 Sep 30;87(19):9576–9579. doi: 10.1021/acs.analchem.5b03432

Figure 2.

Figure 2

Performance of TFAB. (A) SMT peak area was proportional to protein concentration, and repeated thermal scanning confirmed protein stability. Inset image shows microfluidic TFAB in 100 pL channels. (B) Duplex TFAB for insulin and thrombin quantification in 10-fold diluted human serum. (C) Insulin TFAB in 10-fold diluted human plasma (filtered); longer wavelength fluorescence emission (TYE665) was shown to reduce autofluorescence effects as well. (D) Insulin TFAB in cell media. 1 h of insulin secretion is directly quantified from only 7 murine pancreatic islets at low and high glucose.