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. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Anal Chem. 2015 Sep 30;87(19):9576–9579. doi: 10.1021/acs.analchem.5b03432

Figure 3.

Figure 3

Modeling and exploitation of bivalency in TFAB. (A) The TFAB model consisted of two consecutive binding events that were decoupled, represented by Kα and Kβ. (B) A Van’t Hoff plot showing thrombin TFAB processed through the model system. (C) Comparison of novel isothermal bivalent assay (solid line) with its monovalent counterpart (dotted line) showed an order-of-magnitude improvement in dynamic range with bivalent probes.