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. 2015 Oct 12;16:61. doi: 10.1186/s12865-015-0124-x

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© Niu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Cytokine production in NK, CIK, and γδ T cells. a Intracellular staining of IFN-γ and IL-2 in NK, CIK, and γδ T cells. Intracellular levels of IL-2 and IFN-γ were measured as described for perforin and granzyme B detection. b Extracellular cytokine production of NK, CIK, and γδ T cells. The culture supernatants were collected and analyzed by ELISA for IFN-γ, IL-2, IL-4, IL-6, and IL-10. Results are expressed as the mean ± SD, n = 20. * p < 0.05, ** 0.001 < p < 0.01, *** p < 0.001, ns p > 0.05