Table 2.

Brain tissue blocks and stains used for neuropathology

		Stain
		Reserve	Luxol fast blue	Bielschowsky silver stain	AT8	Amyloid-β (4G8)a	α-Synucleina	pTDP-43a	Minimum stainingb
Brain region	Paraffin blocks, n
Olfactory bulb	1		X		X		X		X
Midbrain at level of red nucleus	1		X		X	X	X	X
Midbrain at superior cerebellar peduncle	1	X
Precentral, postcentral cortices (BA 4, 3, 2, 1)	1		X		X
Inferior parietal cortex (BA 39, 40)	1		X	X	X	X			X
Anterior cingulate (BA 24)	1		X				X
Superior frontal (BA 8, 9)	1		X		X				X
Inferior frontal cortex (BA 10, 11, 12)	2		X		X				X
Dorsolateral frontal (BA 45, 46)	2		X	X	X	X		X	X
Caudate-putamen-accumbens, septal cortex	2		X		X	X
Insular cortex	2		X		X
Temporal pole (BA 38)	1		X		X				X
Superior temporal (BA 20, 21, 22)	1		X		X	X			X
Amygdala, with entorhinal cortex (BA 28)	1		X		X	X	X	X	X
Globus pallidus, insula, substantia innominata	1		X		X
Anterior hippocampus	1	X
Hippocampal formation, lateral geniculate	1		X	X	X	X		X	X
Superior temporal posterior (BA 41, 42)	1	X
Thalamus with subthalamic nucleus	1	X
Hypothalamus, mammillary body	1		X		X
Posterior thalamus	1	X
Posterior cingulate (BA 23, 31)	1	X
Calcarine cortex (BA 17, 18)	1		X	X	X
Superior parietal cortex (BA 7B)	1	X
Upper pons (level of locus coeruleus)	1		X		X				X
Pons, middle cerebellar peduncle	1	X
Medulla oblongata with inferior olives	1		X		X		X		X
Cervical spinal cord	1		X		X			X
Thoracic spinal cord	1	X
Lumbar spinal cord	1		X
Sacral spinal cord	1	X
Cerebellar vermis	1		X		X
Cerebellum with dentate nucleus	1		X	X	X	X			X
Parastriate cortex	1	X

Abbreviations: BA Brodmann area, pTDP-43 phosphorylated transactive response DNA binding protein 43 kDa

^aIf screening regions are positive for β-amyloid, α-synuclein, or pTDP-43, additional regions are stained to allow for complete staging of these pathologies

^bMinimum regions that must be evaluated to make a neuropathological chronic traumatic encephalopathy diagnosis