Activity of monomers (○) and polymers (●). (A) Change in CF emission intensity I during the addition of reaction mixtures with and without initiator 5 (50 s, 75 μM final guanidinium (monomer 1) concentration) and excess Triton X100 (300 s) to EYPC-LUVs⊃CF. Reaction mixtures: 100 mM 1, 0 (○) or 5 mM 5 (●), 1 M TEOA, pH 7, 10 min; termination: 5 mM 9. (B) Transport activity Y of 1 before (○) and after polymerization (●, 5 mM 5) with increasing concentration of guanidinium cations (Y = I before lysis in A (~5 min)).