Table 1.

Comparison of the properties of purified recombinant adenine deaminase (Aada6hp), guanine deaminase (Agda6hp), urate oxidase (Auor6hp) and wild-type xanthine oxidoreductase (Axorp)

Property	Aada6hp	Agda6hp	Axorp	Auor6hp
pH optimum	6.5	6.5	8.5	9.5
pH range*	5.5–7.5	6.0–8.0	7.5–9.5	8.0–10.0
pH stability*	7.5–8.5	6.5–8.0	8.0–10.5	8.0–10.0
Temperature optimum	40°C	55°C	43°C	35°C
Temperature range*	34–46°C	40–60°C	35–50°C	30–45°C
Temperature stability/1h*	30°C	30°C	30°C	50°C

^*Temperature, pH range and stability were defined as the temperature and pH at which over 80% of the initial activity was retained. pH stability was measured after incubation for: 24 h for Aada6hp, 4 h for Axorp, 1 h for Auor6hp and Agda6hp.