Figure 1.
In vitro amplification of hamster adapted TME. (A) HY TME and DY TME were diluted in hamster brain homogenate and subjected to 144 cycles of 5-second sonication and 10 minute incubation. Following PK digestions, Western blot analysis show amplification of PrPSc when compared to their unsonicated controls (lanes 3–4 vs. 5-6 for HY TME and lanes 7-8 vs. 9-10 for DY TME). HY TME amplifies more efficiently than DY PrPSc (compare lanes 5-6 vs. 9-10). Mock: mock infected negative control (lanes 11–12). The migration of the 19 and 21 kDa unglycosylated PrPSc polypeptides is indicated on the left of panel A. (B) Bar graph comparing the relative PrPSc intensity before and after PMCA using HY TME or DY TME as a PrPSc seed (n = 5 per experimental group).