Table 1.
Primers and Probes Sequences designed for the Multiplex Nested Real Time PCR assay.
| Primer Name | Sequence (5’-3’)a |
|---|---|
| OliNestCyp51A1 (Forward. First PCR) | CTYGCTCAATGTYGTTTATCAAYTATTCTTYCGGC |
| OliNestCyp51A2 (Reverse. First PCR) | CGCMGACTGAGTYAAGCCGTACTTGATGAACTTC |
| OliCyp51int-3 (Forward, second PCR) | CAGAACCGCCAATGGTCT |
| OliCyp51int-2 (Reverse, second PCR) | GACRTCCTTGAGCTTGCC |
| Cyp51int-AF (A.fumigatus probe) | FAM-CGCGATCAGATTGTAGTTTGACATTCATTCCTGGGGATCGCG-BHQ1 |
| Cyp51int-AL (A. lentulus probe) | HEX-CGCGATCAAGCTAGCGTCTGACATTTATCCCGGGATCGCG-BHQ1 |
a
Underlining regions indicates the PCR target sequence