Table 1.
Strains, plasmids, and primers used in this study. Primer sequences are written 5′→3′
| Description | Source or Reference | |
|---|---|---|
| Strains | ||
| F. tularensis | ||
| LVS | F. tularensis subsp. holarctica live vaccine strain | Karen Elkins |
| E. coli | ||
| 5-α | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | New England Biolabs |
| P. aeruginosa | ||
| 1244 | Wild type | Peter Castric |
| PA14 | Wild type | Costi Sifri |
| Plasmids | ||
| pFNLTP8 | Francisella shuttle plasmid, Kmr | Ref.8 |
| pABST | pFNLTP8 with F. tularensis LVS groE promoter | This study |
| pBR | pABST with P. aeruginosa 1244 pilA under the control of the F. tularensis LVS groE promoter | This study |
| pOPRF | pABST with P. aeruginosa PA14 oprF under the control of the F. tularensis LVS groE promoter | This study |
| pFLI | pABST with P. aeruginosa PA14 fliC under the control of the F. tularensis LVS groE promoter | This study |
| pGRP | Francisella shuttle vector containing the promoter of FTL_0581 | Ref.17 |
| pGFLI | pGRP under the control of the F. tularensis LVS FTL_0581 promoter | This study |
| Primers | ||
| groE1 | ACGTGGTACCCGAGAGCTTGTTTGACAAAAAAAC | This study |
| groE2 | CATGGAATTCAACAATCTTACTCCTTTGTTAAATTATTTTTG | This study |
| pilA1 | ACGTGAATTCATGAAAGCTCAGAAGGGTTTTAC | This study |
| pilA2 | CATGCATATGTTAGGATTTCGGGCAATTAGC | This study |
| oprFfwd | CATGGAATTCCTAACTGACCATCAAGATGGG | This study |
| oprF2rev | CATGCATATGGCCGGGTTTTTCCTTAGAG | This study |
| fliCfwd | CATGGAATTCCGCAAGCTCAGGTAACCGAAATAGGTCCTTTGGAGGAAATC | This study |
| fliCrev | CATGCATATGTTAGCGCAGCAGGCTCAGGACCGCC | This study |
| fliCfwdnde | CATGCATATGCGCAAGCTCAGGTAACCGAAATAGG | This study |
| fliCrevbam | CATGGGATCCTTAGCGCAGCAGGCTCAGGACCGCC | This study |