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. 2015 Apr 1;6(2):121–126. doi: 10.1080/21505594.2015.1009732

Figure 2.

Figure 2.

R. oryzae germlings deprived of iron display typical morphological changes associated with apoptosis, including nuclear degradation (by TUNEL assay), caspase-like activity (by CaspACE FITC-VAD-FMK), and extracellular ATP release (by CellTiter-Glo luminescent assay) in R. oryzae FTR1::RNAi and control strains grown for 24–96 h. Fluorescent images of R. oryzae FTR1::RNAi and control strains grown for 96 h in iron-deprived condition, stained with TUNEL (A), CaspACE FITC-VAD-FMK (B). Extracellular ATP release is higher in R. oryzae FTR1::RNAi mutant than control strain grown in iron-starved and iron-rich conditions, respectively (C). DIC, differential interference contrast). **p <0.001; ***p<0.0001 (compared with control).