Table 1.
Summary of Rho family GTPase accumulation in wildtype and various backgrounds in response to cell wounds
| Rho Accumulation |
Rac Accumulation |
Cdc42 Accumulation |
|||||
|---|---|---|---|---|---|---|---|
| Genotype | Xenopusa | Drosophilab | Xenopusa | Drosophilab | Xenopusa | Drosophilab | |
| WT | Array of active RhoA in ring; interior to and no overlap with active Cdc42 array | Array of Rho1 in ring; partially interior to Cdc42 and Rac arrays; Array of active Rho1 at innermost part of Rho1 ring | ND | Array of Rac in ring & halo; overlapping with Cdc42 array at ring; partial overlap with Rho1 at inner ring edge | Array of active Cdc42 in ring; exterior to and no overlap with RhoA array | Array of Cdc42 in ring; overlapping with Rac at outer ring edge; partial overlap with Rho1 at inner ring edge | |
| Inhibitors/Mutants c | |||||||
| Rho | C3 toxin; LOF mutant | Rho array fails to form | Rho array fails to form | ND | Lower level in array; Elevated background level; array broadens | Increased level in array; array broadens | Array narrows |
| CA | Elevated background level; array formation impaired | ND | ND | ND | Array broadens | ND | |
| Cdc42 | DN; LOF mutant | Array fails to form | ND | ND | ND | Array fails to form | ND |
| CA | Array broadens | ND | ND | ND | ND | ||
| Rac | NSC27366; LOF mutant | ND | Lower level in array | ND | Decreased c | ND | Lower level in array |
| Actin | Jasplakinolide | Outward broadening of array; array does not translocate | Lower level in array; array does not translocate | ND | Lower level in array; array does not translocate; accumulation delayed | Outward broadening of array; rapid turnover; array does not translocate | Lower level in array; array does not translocate; accumulation delayed; array is narrower |
| LatrunculinB; Concanavalin A | Elevated background level; array broadens; array does not translocate | ND | ND | ND | Decreased background level; array broadens; array does not translocate | ND | |
| Myosin | Y27632 ; Blebbistatin | Array translocation rate slowed | Lower level in array; array does not translocate; array broadens | ND | Lower level in array; array does not translocate; accumulation delayed | Array translocation rate slowed | Lower level in array; array does not translocate; accumulation delayed |
a
References cited.13,21,22,24
b
References cited.17,20
c
ND: Not Done; WT: wildtype; LOF: loss-of-function; CA: constitutively active; DN: dominant negative.`
d
NSC27366 cannot be concentrated to levels sufficient to remove Rac1-GFP in addition to endogenous Rac1, Rac2, and MTL.