Figure 3.

ArchCandy prediction of the effect of amino acids substitutions on Sup35p fibril-forming potential. (A) Wild type Sup35p. The fibril-forming potential is measured by the number of predicted β-arcades (with ArchCandy score above 0.575) for each amino acids in Sup35p N-domain. For example, residue 23 of wild type Sup35p can participate in 37 different β-arcades. Panels B–E compare ArchCandy predictions for modified Sup35 proteins (continuous lines) and the wild type protein (dashed line). For proline substitutions and glycine insertions only some typical results are shown. Each plot is labeled by corresponding amino acid substitution(s) or sup35 allele. The data used are from Refs. 21, 22, 26 and 31.