Table 2. Comparison of the kinetic parameters obtained for three representative nickel(II)-dependent enzymes.

One international unit (IU) is defined as the production of 1mol of S-D-lactoylglutathione product per minute.

Enzyme	K m, HTA (M)	V max (IUmg1)
Z. mays †
Trace-metals, 100M nickel(II)	56.0 5.0	43.3 1.1
Trace-metals, 100M cobalt(II)	32.3 2.3	12.3 0.2
Ni-only, 100M nickel(II)	40.5 3.2	87.5 1.2
Trace-metals, as purified	58.0 8.2	3.2 1.2
His6-less, 100M nickel(II)	39.2 4.2	90.1 2.5
O. sativa ‡	99.8 1.3	131 12
E. coli §	27.0 0.4	676 17

^†This work.

^‡ Mustafiz et al. (2014 ▸).

^§Clugston et al. (2004 ▸).