Table 4. Data-processing statistics of lysozyme crystals studied with the HC1b.
A comparison between the controls, lysozyme and lysozyme wrapped in graphene/PMMA, with an unwrapped lysozyme crystal and a lysozyme crystal wrapped in graphene/PMMA exposed to a relative humidity of 70%. Although only one data set is shown for each sample, at least three crystals were collected for each scenario; the statistics from all of the crystals can be found in the Supporting Information. All data sets showed a similar trend for each scenario, except in the case where a relative humidity of 70% was applied to an unwrapped crystal. In this case seven data sets were collected, all of which showed an increase in the values of R meas and the B factor, and in two of the seven data sets a reduction in the unit cell was also observed. Values in parentheses are for the outer shell.
| Lysozyme | Graphene/PMMA-wrapped lysozyme | Lysozyme exposed to 70% RH | Graphene/PMMA-wrapped lysozyme exposed to 70% RH | |
|---|---|---|---|---|
| Graphene/PMMA | X | X | ||
| Relative humidity applied at 70% | X | X | ||
| Space group | P43212 | P43212 | P43212 | P43212 |
| Unit-cell parameters () | a = b = 78.0, c = 37.1 | a = b = 77.8, c = 38.0 | a = b = 74.3, c = 33.6 | a = b = 78.1, c = 37.4 |
| Resolution range () | 34.891.70 (1.741.70) | 34.141.70 (1.751.70) | 30.642.84 (2.992.84) | 33.701.70 (1.741.70) |
| Total No. of reflections | 157599 (9480) | 157565 (9084) | 28043 (4185) | 160618 (9573) |
| No. of unique reflections | 13018 (908) | 13281 (946) | 2475 (347) | 13233 (942) |
| Completeness (%) | 99.4 (96.8) | 99.9 (99.3) | 99.9 (100) | 99.9 (99.8) |
| Multiplicity | 12.1 (10.4) | 11.9 (9.6) | 11.3 (12.1) | 12.1 (10.2) |
| I/(I) | 45.1 (17.9) | 48.5 (29.6) | 18.3 (2.0) | 46.3 (14.2) |
| R meas | 0.040 (0.104) | 0.048 (0.065) | 0.076 (1.427) | 0.034 (0.137) |
| Overall B factor from Wilson plot (2) | 14.0 | 14.4 | 109.9 | 18.4 |