Figure 2.

Numerous genes expressed in native Ewing sarcoma tumors are down-regulated in tumor cell lines cultured in monolayers. The fold change in gene expression was normalized to actin levels in the individual samples and then to the corresponding levels in cells cultured in monolayer. Data are shown as Average ± SD (n = 3–5). Two-tailed Student´s T-test was used to determine statistical significance.*P < 0.05; **P < 0.01, ***P < 0.001; ns, not significant (Reproduced with permission from Villasante et al. Biomaterials 35: 5785-5794, 2014).