Figure 5.

Fluorescence spectroscopy of thioflavin T binding to Nab3¹³⁴ and Aβ. (A) Thioflavin T was mixed with the indicated proteins (2 μmolar each, except BSA which was 20 μM) and the spectra of fluorescence was scored in a spectrofluorometer in triplicate and averaged. Error bars show the standard deviation around those averages. (B) Increasing amounts of Nab3¹³⁴ protein were added to thioflavin and absorbance at 485 nm was quantified and plotted. The correlation coefficient (R²) was calculated with Prism 6.02 software (GraphPad).