Figure 3.

The ability of Zuo1 to associate with ribosomes and stimulate Ssb is required for suppression of prion formation. (A) Wildtype Zuo1, a mutant lacking the J domain required for Ssb ATPase activation (zuo1Δ111–165), and a mutant lacking the charged region required for ribosome association (zuo1Δ284–363) were examined for their ability to block prion formation in vivo. (B) Frequency of induced [psi⁻] to [PSI⁺] conversion following transient over-expression of the Sup35 N and M domains for Δzuo1 and wildtype strains ectopically expressing different Zuo1 constructs. Percentage Ade⁺ was calculated as described for Figure 1A. Error bars represent 95% confidence intervals. **** p < 0.0001 (2-proportion z-test).