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. 2015 Jan 13;6(2):132–144. doi: 10.4161/21505594.2014.989795

Figure 8.

Figure 8.

Detection of PrPSc in the brains of hamsters from the first and second passage (data from the first reconstitution experiment). (A) Brain PrPSc profile of infected animals from first reconstitution experiment. Inocula 9 (lane 3), 8.1 (lane 4; 151 dpi) and 8.2 (lane 5; 246 dpi). Lane 1: Positive control from 263K scrapie hamster; lane 2: control sample from healthy hamster. (B) Brain PrPSc profile of infected animals from first reconstitution experiment. Inocula 2* (lane 3; environmentally contaminated animal), 6 (lane 4), 7 (lane 5), 11 (lane 6) and 15 (lane 7). Positive control from 263K scrapie hamster; lane 2: control sample from healthy hamster. A selection of animals that died of causes not related to scrapie or deliberate infection are represented here. (C) First passage: WB profile of 2 distinct hamsters inoculated with BH of animal 8.1 (lanes 3 and 4); WB profile of 2 distinct hamsters inoculated with BH of animal 8.2 (lanes 5 and 6).