Table 1.
Mouse-adapted field scrapie isolates used in this study
| Case | Breed (PrP genotype)b | Isolate or strain (Phenotype) | Passage history in ICR mice | Incubation time | Transmissibility to GT1–7 | Transmissibility to L929 |
|---|---|---|---|---|---|---|
| Natural | Suffolk (NT c) | Obihiro (Short-type) | Serial | 141 ± 11.7 (8/8 d) | – | +e |
| Natural | Corriedale (NT) |
Tsukuba-1 (Short-type) |
1st 2nd 3rd |
213 ± 16 (6/6) 180 ± 8.0 (6/6) 133 ± 2.0 (6/6) |
NT NT – |
NT NT +e |
| Natural | Suffolk (NT) |
Tsukuba-2 (Long-type) |
1st 2nd 3rd |
359 ± 21 (6/6) 299 ± 1.0 (6/6) 288 ± 5.0 (6/6) |
NT NT + |
NT NT NT |
| Natural | Suffolk (ARQ) |
Tok (Short-type) |
1st 2nd 3rd |
295 ± 40 (11/11) 146 ± 1.0 (7/7) 146 ± 5.0 (10/10) |
NT NT – |
NT NT + e |
| Natural | Suffolk (ARQ) |
Tok-S24 (Short-type) |
1st 2nd 3rd |
426 ± 35 (6/6) 138 ± 1.0 (7/7) 146 ± 13 (7/7) |
NT NT – |
NT NT NT |
| Natural | Suffolk (ARQ) |
Tok-S34 (Short-type) |
1st 2nd 3rd |
268 ± 24 (9/9) 155 ± 8.0 (5/5) 147 ± 0.5 (7/7) |
NT NT – |
NT NT NT |
| Natural a | Suffolk (ARQ) |
Ka/W (Short-type) |
1st 2nd 3rd |
457 ± 21 (15/16) 256 ± 29 (5/5) 152 ± 5.6 (10/10) |
NT NT – |
NT NT + |
| Ka/O (Long-type) |
1st 2nd 3rd |
469 (1/16) 287 ± 6.5 (5/5) 272 ± 29.0 (10/10) |
NT NT + |
NT NT + |
||
| Natural | Suffolk (ARQ) |
Fukuoka (Short-type) |
1st 2nd |
442 ± 62.4 (20/20) 155 ± 4.4 (10/10) |
– – |
NT + |
| Natural | Suffolk (ARQ) |
Oita (Long-type) |
1st 2nd |
447 ± 33.7 (20/20) 279 ± 19.7 (10/10) |
+ + |
NT + |
| Laboratory strain | ME7 | Serial | 143 ± 4.4 | − | + | |
| Laboratory strain | Chandler | Serial | 135 ± 5.6 | + | NT | |
| Laboratory strain | 22L | Serial | 138 ± 5.0 | + | +e |
a
Two biologically different prions were isolated from one sheep. Sixteen mice were inoculated with scrapie-affected sheep brain homogenate. Only one mouse in the line of Ka/O first passage exhibited polydipsia, polyuria, and obesity as clinical signs while the others (15/16) did not show these clinical signs.
b
PrP amino acid sequence at codons 136, 154, and 171.
c
Not tested.
d
Number of sick mice/total inoculated mice.
e
Data not shown.