FIGURE 3.
In vitro neutralization efficacy of polyclonal PrPSc-specific ovine antibodies in trivalent formulation. Polyclonal antibody for each disease-specific epitope was purified from immune serum from sheep receiving multiple doses of recombinant Lkt-YYR, -YML, or -RL antigen (Hedlin, 2010; Marciniuk, 2014). L929 cells were co-cultured with (A) 0.01, (B) 0.1, (C) 1.0, or (D) 10 μg of YYR, YML, and RL pAb in combination (Trivalent) and 0.01% – 0.00001% RML brain homogenate for 5 days in 96 well culture plates. Exposed cells were passaged 3 times with 20,000 cells collected at the third passage and loaded on to filter plates. Results are expressed as percent inhibition (Control – Treatment / Control *100) + SEM of values from replicate assays.