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The active site for the phosphotransfer reaction. (a) RPA3017 (green) is positioned with the DHp domain of HK (blue) according to the framework of the HK853–RR468 complex structure (PDB entry 3dge). The active-site residues of RPA3017 are shown as yellow sticks, while His532 of RpBphP2 is shown in cyan. (b) His532 of RpBphP2, the phosphoryl group (indicated by a sulfate group) and Asp70 of RPA3017 align to carry out inline nucleophilic attack in the phosphotransfer reaction.
