Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Method | Hanging drop |
| Plate type | 24-well VDX plate |
| Temperature (K) | 293.15 |
| Protein concentration (mgml1) | 15 |
| Buffer composition of protein solution | 25mM TrisHCl pH 7.5, 100mM NaCl, 3mM DTT, 10% glycerol |
| Composition of reservoir solution | 100mM sodium acetate pH 4.6, 100mM CdCl2, 21% PEG 400 |
| Volume and ratio of drop | 4l, 1:1 ratio |
| Volume of reservoir (ml) | 1 |
