Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Method | Sitting drop |
| Plate type | Cryschem, 24-well, Hampton Research |
| Temperature (K) | 279 |
| Protein concentration (mgml1) | 21.6 |
| Buffer composition of protein solution | 50 mM HEPES pH 7.4, 150mM NaCl |
| Composition of reservoir solution | 14% PEG 4000, 0.133M NH4NO3 |
| Volume and ratio of drop | 1l:1l |
| Volume of reservoir (l) | 200 |
