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| Method | Hanging-drop vapour diffusion |
| Plate type | 24-well plates, Hampton Research |
| Temperature (K) | 293 |
| Protein concentration (mgml1) | 14 |
| Buffer composition of protein solution | 20mM TrisHCl pH 8.0, 150mM NaCl |
| Composition of reservoir solution | 2.4M sodium chloride, 0.1M magnesium chloride, 0.1M TrisHCl pH 7.1 |
| Volume and ratio of drop | 2l, 1:1 |
| Volume of reservoir (l) | 300 |
