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| Method | Hanging-drop vapor diffusion |
| Plate type | Neuroprobe 96-well |
| Temperature (K) | 293 |
| Protein concentration (mgml1) | 14 |
| Buffer composition of protein solution | 20mM TrisHCl, 100mM NaCl pH 8.0 |
| Composition of reservoir solution | 100mM MMT pH 5, 22.2%(w/v) PEG 2000 MME |
| Volume and ratio of drop | 0.4l:0.4l |
| Volume of reservoir (l) | 500 |
