Table 1. Composition of P22 C1 and expression strategy.

Restriction sites in the forward and reverse primers are underlined.

Source organism	Phage P22
DNA source	Genomic DNA
Forward primer	GAATTCCATATGGAACTCACAAGCACTCGCAAGA
Reverse primer	CGCGGATCCTCAGGCCTCAAAACTGTTCCC
Cloning and expression vector	pET-28a
Expression host	E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced†	GSHMELTSTRKKANAITSSILNRIAIRGQRKVADALGINESQISRWKGDFIPKMGMLLAVLEWGVEDEELAELAKKVAHLLTKEKPQDCGNSFEA

^†C1 in pET-28a after removing the His tag.