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. 2015 Sep 23;71(Pt 10):1286–1291. doi: 10.1107/S2053230X15015708

Table 2. Crystallization conditions.

Method Vapour diffusion
Plate type 96-well
Temperature (K) 293
Protein concentration (mgml1) 24
Buffer composition of protein solution 20mM Tris pH 8.0, 300mM NaCl
Composition of reservoir solution 0.1M HEPES pH 7.0, 10% PEG 6000, 0.01M MgCl2, 0.08M magnesium acetate
Volume and ratio of drop 2l, 1:1
Volume of reservoir (l) 500