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. 2015 Sep 23;71(Pt 10):1300–1308. doi: 10.1107/S2053230X15016064

Table 1. SepL production information.

Source organism E. coli
DNA source E. coli K12
Forward primer CACAGCAGCGGCCTGGTGCCGCGCGGCAGCGCTAATGGTATTGAATTTAATCAAAACC
Reverse primer GGTGGTGGTGGTGGTGCTCGAGTTCATCACATAACATCCTCCTTATAATCTATCAC
Cloning vector pET-28a/pET-21a
Expression vector pET-28a/pET-21a
Expression host E. coli
Complete amino-acid sequence of the construct produced GSARKEEEGTTIEKLLNEMQELLTLTDSDKIKELSLKNSGLLEQHDPTLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYAKLLLDLLGEDDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPEREKHLKVIIRALSFDLSYMSSFEDKVRTSSIISDLCRIIIFLSLNNYTDIIAISIKKDKDVILNEMLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKEFFASLPDACFIDNEQRSNTLLMIGKVIDYKEDVM