Table 1. SepL production information.
Source organism | E. coli |
DNA source | E. coli K12 |
Forward primer | CACAGCAGCGGCCTGGTGCCGCGCGGCAGCGCTAATGGTATTGAATTTAATCAAAACC |
Reverse primer | GGTGGTGGTGGTGGTGCTCGAGTTCATCACATAACATCCTCCTTATAATCTATCAC |
Cloning vector | pET-28a/pET-21a |
Expression vector | pET-28a/pET-21a |
Expression host | E. coli |
Complete amino-acid sequence of the construct produced | GSARKEEEGTTIEKLLNEMQELLTLTDSDKIKELSLKNSGLLEQHDPTLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYAKLLLDLLGEDDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPEREKHLKVIIRALSFDLSYMSSFEDKVRTSSIISDLCRIIIFLSLNNYTDIIAISIKKDKDVILNEMLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKEFFASLPDACFIDNEQRSNTLLMIGKVIDYKEDVM |