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| Method | Microbatch |
| Temperature (K) | 300 |
| Protein concentration (mgml1) | 20 |
| Buffer composition of protein solution | 20mM HEPES pH 7.5, 500mM NaCl |
| Composition of screen solution | 0.1M SPG buffer pH 6, 25% PEG 1500 + 0.1M Tris pH 8.5 and 3M NaCl additive |
| Volume of drop (l) | 0.6 |
