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| Method | Vapour diffusion |
| Plate type | Sitting-drop vapour diffusion |
| Temperature (K) | 285 |
| Protein concentration (mgml1) | 11 |
| Buffer composition of protein solution | 25mM Tris pH 9.0, 50mM NaCl, 2mM PMSF |
| Composition of reservoir solution | PEG 1500 [21, 23, 25, 28 or 30%(w/v)] and buffer pH 6.0, 7.0, 8.0 or 9.0 |
| Volume and ratio of drop | 1l:1l |
| Volume of reservoir (l) | 500 |
