Table 1. Macromolecule-production information.
Source organism | T. elongatus |
DNA source | T. elongatus genomic DNA |
Forward primer | TTCCGCGTGGATCCGTGGATAACGTCATC |
Reverse primer | CTGCAGATCTCGAGTTAGTACAGCTGG |
Expression vector | pRSET A |
Expression host | E. coli (KRX) |
Complete amino-acid sequence of the construct produced | MRGSHHHHHHGLVPRGSMDNVIGLEIIEVVEQAAIASARWMGKGDKNMADQAAVDAMRNRMNQIHMRGRIVIGEGERDEAPMLYIGEEVGICTRPDAAQYCNPEELIEIDIAVDPCEGTNLCAYGQPGSMAVLAISEKGGLFAAPDFYMKKLAAPPAAKGKVDIRNSATENLKILSECLDRAIDELVVVVMKRDRHNDLIQEIRDAGARVQLISDGDVSAALACAFSGTNIHALMGIGAAPEGVISAAAMRALGGHFQGQLVYDPAVVMTKEWANRTREGNLEELKKAGITDPDKVYEAEELASGETVLFAACGITPGMLMKGVRFFKGGARTQSLVISTQSKTARFVDTIHMFDQQLKSLQLY |