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. 2015 Sep 23;71(Pt 10):1341–1345. doi: 10.1107/S2053230X15016829

Table 1. Macromolecule-production information.

Source organism T. elongatus
DNA source T. elongatus genomic DNA
Forward primer TTCCGCGTGGATCCGTGGATAACGTCATC
Reverse primer CTGCAGATCTCGAGTTAGTACAGCTGG
Expression vector pRSET A
Expression host E. coli (KRX)
Complete amino-acid sequence of the construct produced MRGSHHHHHHGLVPRGSMDNVIGLEIIEVVEQAAIASARWMGKGDKNMADQAAVDAMRNRMNQIHMRGRIVIGEGERDEAPMLYIGEEVGICTRPDAAQYCNPEELIEIDIAVDPCEGTNLCAYGQPGSMAVLAISEKGGLFAAPDFYMKKLAAPPAAKGKVDIRNSATENLKILSECLDRAIDELVVVVMKRDRHNDLIQEIRDAGARVQLISDGDVSAALACAFSGTNIHALMGIGAAPEGVISAAAMRALGGHFQGQLVYDPAVVMTKEWANRTREGNLEELKKAGITDPDKVYEAEELASGETVLFAACGITPGMLMKGVRFFKGGARTQSLVISTQSKTARFVDTIHMFDQQLKSLQLY