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| Method | Hanging-drop vapour diffusion |
| Plate type | 24-well plate |
| Temperature (K) | 290 |
| Protein concentration (mgml1) | 15 |
| Buffer composition of protein solution | 50mM TrisHCl pH 7.9, 50mM NaCl, 1mM MgS7P, 0.2M MgCl2 |
| Composition of reservoir solution | 0.06M Na HEPES pH 7.5, 0.12M MgCl2, 27%(v/v) PEG 400 |
| Volume and ratio of drop | 2l (1:1) |
| Volume of reservoir (l) | 100 |
