Fig. 6.

Expression of primary ciliary markers in the feeding centers in Ankrd26−/− and WT mice. a, b Show immunostaining of AC3 in the PVN of the WT (a) and Ankrd26−/− (b) mice. Note the almost absent immunolabeling in the PVN of the Ankrd26−/− mice. c, d The number of AC3-positive primary cilia is comparable in the ARC in WT (c) and Ankrd26−/− (d) mice. Scale bar 60 µm. Asterisk indicates the third ventricle. e–f IHC of Mchr1 (green) in the PVN in WT and Ankrd26−/− mice. e Neurons of the PVN express Mchr1 in the primary cilia (arrowhead) and in the cytoplasm (arrow). f Ankrd26−/− mice lack Mchr1-positive primary cilia in this region, but the immunostaining is present in the cytoplasm (arrow). Scale bar 10 µm. g–l Expression of Sstr3 (green) and AC3 (red) in the VM nucleus in WT (g–i) and in Ankrd26−/− (j–l) mice. g, j In WT mice (g) Sstr3 is localized to the primary cilia (arrows) and to the cytoplasm, while in the Ankrd26−/− mice (j) the ciliary staining is almost absent. h, k Numerous AC3-positive primary cilia are present in the WT mice (h), while reduced number and shorter AC3-positive primary cilia can be seen in the Ankrd26−/− mice (k). i, l Merged images indicate numerous Sstr3 and AC3 co-labeled primary cilia in the WT mice (i). In Ankrd26−/− mice (l) the number of Sstr3 and AC3-positive primary cilia is markedly reduced. Scale bar 15 µm. Nuclei are visualized with DAPI (blue). Images are representative of four independent observations (n = 3 in each group, four different litters)