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. 2015 Jan 19;20(1):1643–1660. doi: 10.3390/molecules20011643

Figure 1.

Figure 1

In vitro reconstitution of the human full-length Hsp90β:p50Cdc37 complex and phosphorylation by CK2α. (A) Elution profiles and corresponding SDS-PAGE for analytical gel filtration experiments showing complex formation (blue) upon incubation of free Hsp90β (red) with free p50Cdc37 (green). (B) Coomassie-stained SDS-PAGE and Western blot analysis of purified Hsp90β in the presence and absence of CK2α and ATP using anti-Hsp90β (pSer255); (C) Coomassie-stained SDS-PAGE and Western blot analysis of purified p50Cdc37 in the presence and absence of CK2α and ATP using anti-p50Cdc37 (pSer13); (D) Phosphorylation states of Ser255 in Hsp90β upon phosphorylation by CK2α and dephosphorylation by alkaline phosphatase (AP).