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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Anal Biochem. 2014 Oct 14;470:14–21. doi: 10.1016/j.ab.2014.10.001

Figure 3.

Figure 3

Influence of Cell Plating Number on Transfection in 384 and 1536-Well Format. Panel A illustrates the result of plating HepG2 cells (25 μL, 2500-20000 cells) in 384-well plates and transfecting with 5 μL (0-300 ng, PEI gWiz-Luc, N:P = 9). At 48 hr post-transfection, 10 μL of ONE-Glo was added and the RLU was measured after 5 min (n = 3). Panel B illustrates the result of transfection of 0-120 ng (2 μl) of PEI gWiz-Luc (N:P 9) into HepG2 cells (600-1500 cells in 6 μL per well) in 1536-well plates. At 48 hr, 3 μL was removed and 2 μL of ONE-Glo was added the bioluminescence data was measured at 25 min (n = 4). * indicates p ≤ 0.05 relative to 0 DNA dose.