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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Anal Biochem. 2014 Oct 14;470:14–21. doi: 10.1016/j.ab.2014.10.001

Figure 8.

Figure 8

PEI and CaPO4 Transfection of Primary Hepatocytes in 384-Well Plates. Panel A illustrates the transfection of 250 primary hepatocytes (45 μL) per well with 0-750 ng of PEI gWiz-Luc (N:P 7). At 24 hr 10 μL of ONE-Glo was added and bioluminescence was measured at 5 min (n = 9). Panel B illustrates the transfection of primary hepatocytes (45 μL) plated in 384-well plates at 250 cells per well with 250 ng of CaPO4 calcium phosphate. At 24 hr 10 μL of ONE-Glo was added and bioluminescence was measured at 5 min (n = 9). * indicates p ≤ 0.05 relative to 0 DNA dose.