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. Author manuscript; available in PMC: 2016 Jan 31.

Published in final edited form as: Anal Biochem. 2014 Oct 14;470:14–21. doi: 10.1016/j.ab.2014.10.001

Table 1.

Summary of Optimized Transfection Parameters for Cells on 384 and 1536-Well Plates.

Plate	384-Well Plate			1536-Well Plate
	Cells/well	DNA dose ng/well	S:B^a Ratio	Cells/well	DNA dose ng/well	S:B^a Ratio
HepG2	5000-15000	150-300	2000	900-1200	40-100	400
NIH 3T3	2500-5000	200-300	3800	400-900	60-120	750
CHO	2500-10000	100-300	3200	600-1200	40-120	500
Volume^b	25 μL			6 μL
Polyplex	5 μL			2 μL
ONE-Glo	10 μL			2 μL

^a

Signal to Baseline ratio for luciferase expression.

^b

Represents the volume of media in the assay.